Effect of Culture Medium on Hyoscyamine Production from Four Datura Sp Hairy Roots
نویسندگان
چکیده
Tropane alkaloids present a great economic value in particular for pharmaceutical industry. The culture of Datura sp hairy roots obtained by inoculation with A4 Agrobacterium rhizogenes offers promising prospects for alkaloids in-vitro production. The objective of our study is to try optimizing hyoscyamine production exploiting the composition of mineral fraction of the culture media. On the whole of obtained hairy roots lines, those selected are DT10, DS18, DI3 and DF11 resulting respectively from the genetic transformation of Datura tatula, D. stramonium, D. innoxia and D. ferox. The most effective line is DT10. The obtained results show that the complete MS and B5 media give the best biomass; thus, the diluted media (1⁄2MS, 3⁄4MS, 1⁄2B5 and 3⁄4B5) are more interesting for hyoscyamine production
منابع مشابه
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The (-)-hyoscyamine, atropine and scopolamine (hyoscine) are three valuable tropane alkaloids while scopolamine is the most important member of this group for the pharmaceutical industry due to its higher demand compared to hyoscyamine and atropine. Scopolamine is an anticholinergic reagent with several therapeutic applications. In the current study, the hairy roots culture of Daturametel was u...
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